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根据一份报告，比一根人类头发还细的微针可以在皮肤中释放有效剂量的流感疫苗。Richard Compans及其同事用流感疫苗包裹了不锈钢微针，并把它们插入了小鼠的皮肤中几分钟。在注射了这种疫苗的两周之后，接受微针免疫的组表现出了与通过皮下注射针头注射疫苗的小鼠类似的健壮的免疫应答。 这组作者在注射疫苗的一个月之后为5组对照组和疫苗组小鼠（每组6只）注射了高剂量的流感病毒，结果发现所有对照组的小鼠都死于流感，而接种疫苗组的所有小鼠——无论是通过微针注射还是皮下注射针头注射——都活了下来。两种疫苗组的小鼠都表现出了几乎相同的对流感病毒的免疫应答。这组作者得出结论说，微针释放的疫苗再加上更加容易的接种可能让这种技术成为传统疫苗注射方法的一个有吸引力的替代方案。 推荐原始出处： PNAS，doi: 10.1073/pnas.0812652106，Qiyun Zhu，Richard W. Compans Immunization by vaccine-coated microneedle arrays protects against lethal influenza virus challenge Qiyun Zhua,1, Vladimir G. Zarnitsynb,1, Ling Yea,1, Zhiyuan Wena, Yulong Gaoa, Lei Pana, Ioanna Skountzoua, Harvinder S. Gilla, Mark R. Prausnitzb,2, Chinglai Yanga,2 and Richard W. Compansa,2 Influenza prophylaxis would benefit from a simple method to administer influenza vaccine into skin without the need for hypodermic needles. In this study, solid metal microneedle arrays (MNs) were investigated as a system for cutaneous vaccine delivery using influenza virus antigen. The MNs with 5 monument-shaped microneedles per array were produced and coated with inactivated influenza virus A/PR/8/34 (IIV). As much as 10 μg of viral proteins could be coated onto an array of 5 microneedles, and the coated IIV was delivered into skin at high efficiency within minutes. The coated MNs were used to immunize mice in comparison with conventional intramuscular injection at the same dose. Analysis of immune responses showed that a single immunization with IIV-coated MNs induced strong antibody responses against influenza virus, with significant levels of hemagglutination inhibition activities (>1:40), which were comparable to those induced by conventional intramuscular immunization. Moreover, mice immunized by a single dose of IIV coated on MNs were effectively protected against lethal challenge by a high dose of mouse-adapted influenza virus A/PR/8/34. These results show that MNs are highly effective as a simple method of vaccine delivery to elicit protective immune responses against virus infection. (责任编辑：Doctor001) |
